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Exact(9)
With FF-NDMA, decoding of a single-control signaling per CP in which transmission was attempted is required.
Recall that only one decoding per CP in which a packet was transmitted is needed in FF-NDMA.
Conversely, with FF-NDMA, decoding is needed per CP (i.e., 1 decoding every R time slots) to receive ACK only at those CPs in which a packet has been transmitted (see T2 in (24)).
For example, if the system to be stable has to satisfy π K ≤0.1 then larger values of σ are available to meet the stability condition by using several time slots per CP.
Therefore, FF-NDMA is feedback-free in the sense that it is not needed to broadcast information related to the number of repetitions and to the state of the forthcoming time slots (as in NDMA or H-NDMA) but, in contrast to [32, 33], ACK feedback to acknowledge a correct detection of the devices' packets per CP is assumed.
In experiments 1 4 a total of 32 control participants (two per CP participant) were selected to match the age of CP participants and also gender in most cases.
Similar(51)
The antibodies were obtained from BD Biosciences (San Jose, CA) and included: HLA/DR/DP/DQ FITC: catalog no.: 555558, HLA-A,B,C Phycoerythrin: catalog no.: 555553, CD10 (APC) BD catalog no.: 340923, CD19 (APC-H7) clone: SJ25C1 BD catalog no.: 560177, CD34 (Per CP-Cy5.5) BD catalog no.: 347203, and CD45 (V450) clone: H130 BD catalog no.: 560367.
After 6 7 days, proliferating CD4+ (CD3+CD8−) and CD8+ (CD3+CD8+) T cells were determined by direct staining with mAbs conjugated with α-CD3-Per-CP and α-CD8-PE.
Cells were washed 3 times with chilled PBS and then incubated with secondary antibody conjugated with R-phycoerythin or Peridinin chlorophyll protein (R-PE or Per-CP, 1 µg) in 50 µl of PBS with 10% FBS for a further 30 minutes at 4°C in the dark.
For CXCR4 detection, 10 μl of PE-conjugated anti CXCR4 (BD, clone 12g5) was used in combination with anti CD45 Per-CP and CD19 Pe-Cy7.
The antibodies employed were CD117-APC/Alexa Fluor750 (immature myeloid cells); CD34-FITC (hematopoietic stem cells); CD45-Per-CP (pan leukocyte marker) and Gr-1-APC/Alexa Fluor750 (immature myeloid cells) (Biosciences Pharmingen, San Diego, CA, USA).
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