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Cell oxygen consumption was calculated by analyzing the slope of the line on the chart recorder and was expressed as nanomoles of oxygen consumed per minute per cell mass.
During phase (I), an exponential glucose flow was imposed to give a constant specific growth rate defined as the variation of cell mass per unit time per cell mass.
Compared to the + N condition, the FAME or TAG content per cell mass increased by five times in the − N case (p <0.05), demonstrating that the additional lipids produced during N limitations were mostly TAGs.
Specific production of compound rates (defined as the variation of compound mass per unit time per cell mass) were calculated considering catalytic biomass concentration and named catalytic specific production rate (q*).
Similar(56)
Hence, it seems that a careful comparative analysis of the calibration steps, as well as a direct measurement of the protein mass per cell volume, will clarify the underlying reasons for the mismatch.
The values of characteristic cell parameters used here for the calculations of protein content per cell dry mass or per cell volume are mostly based on physiological studies from decades ago.
Conclusion: Taking into account the reduced oxygen consumption of the shrinking kidneys, the normal RMR suggests an increased energy expenditure per body cell mass.
However, the ratio of starch consumption per total cell mass between the first and last cycles was 1 to 0.77, showing that major of the displayed α-amylase retained activity after 50 h.
When β cells are exposed to the diabetic state, they become degranulated such that in some experimental systems the content per β cell mass can be reduced to lower than 5% of normal.
Lipid content was expressed as gram lipid per gram dry cell mass, and lipid coefficient as gram lipid produced per gram substrate consumed.
Based on the bacterial growth in the presence and absence of PHMB-functionalized Ag NPspecificpecific growth rate (increase in cell mass per unit time, μ (per hour)) and doubling time td (hours) for E. coli were also calculated (Table 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com