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The fidelity of transcript detection is the key to this "per cell" based normalization method, which generates transcriptome data in "mRNA copy numbers per cell".
Single step growth curves were performed using an initial multiplicity of infection (m.o.i). of 3 TCID50 per cell, based on the titre in CRFK cells.
We based our calculations on the assumption that albumin levels in the GS4.1 cells are comparable with those in hepatocytes in vivo and on information about the copy number of HCV RNA in those cells, which was estimated to be about 1000 per cell based on northern blot analysis [5].
TPGs were calculated per cell, based on protein amount and cell numbers applied.
Hence, Ubb, showing the maximum number of transcripts per cell based on oligoarrays (9,705) had only 401 and 435 transcripts per cell based on MPSS and SAGE, respectively (Table 2).
Infections were performed at a multiplicity of 10 infectious units per cell based on the MCF7 titer.
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This produced a mean true positive estimate of 6.5 insertions per glial cell, based on the PCR validation rate determined for hippocampal neurons (45.0%).
Assuming a transfection efficiency of ~10%, the fold-changes for the transfected cells are ~33-fold and 12-fold on a per cell basis (based on ILMN_2675078).
First, the system examines the percentage of users per femtocell L cell based on its users' capacity: Fig. 3 Power user balancing algorithm scheme {L}_{mathrm{cell}}=frac{N_{mathrm{connected}_mathrm{users}}^{mathrm{cell}}}{N_{mathrm{femtocell}_mathrm{capacity}}} (10).
Assuming 6.7fg P per stationary phase cell (based on an estimate for E. coli; [47]), the potential P contribution from cells was 300 times smaller than the amount of P added externally as NaH2PO4, suggesting that the potential phosphorus contribution from the cells was negligible.
We did not find consistent differences in the staining intensity or per cent positive cells based on grade of the tumour.
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