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Analysis of variance was also used to test the effect of site (a fixed effect with 3 levels: forested, deforested, or lowland) and donor child (a random effect) on the average (per cage) time to sporozoite detection, the average (per cage) oocyst load, or the infection rate.
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Extend the shared cage time.
We made daily observations on survival (number of individuals surviving per cage) and developmental time (mean number of days to complete development from egg to pupa per cage).
Most birds need outside-of-cage time.
In general, four mice (one mouse per cage) were exposed at the same time.
Animals were kept with 4 6 animals per cage until one week before surgery, at which time they were transferred to individual cages (25×25×35 cm).
For longer survival times, rats were housed two per cage and cages were placed on a 37°C heating pad overnight.
We placed each group of six mice for the 24-hr and 28-day sacrifice time points in metabolism cages (two per cage) and performed mass balance studies.
Ten birds per cage was the largest group size in furnished cages at the time of the study.
Three-month-old, adult female Sprague Dawley rats (Charles River), 225 − 250 g at the time of surgery, were housed two to three per cage.
Mice were maintained under clean housing conditions at all times with no more than 5 mice per cage.
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