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We calculated the per bp rate of NCO after applying three broad filters, designed to minimize errors.
Confocal microscopy, differential scanning calorimetry, XRD, and solid-state NMR spectroscopy confirm BP-rich domains with transmembrane-oriented BPs and three to four lipid molecules per BP.
Mean is the mean coverage per bp, i.e., 20% of the sampled bp has up to 11 overlapping reads.
The SNP densities were calculated by determining the number of SNPs per bp of each gene, and the differences between distinct clades were analysed using the nonparametric Wilcoxon test of SPSS 20.0 software (SPSS Inc., Chicago, IL, USA).
"However, BP's deal mechanics are materially better than we anticipated". Jason Gabelman of Cowen & Co. figures the deal to be worth no more than $2 per BP share.
It is of interest that dsDNA binding to the wild-type SsoRadA protein saturated at ∼one protein per bp.
We estimated PCR error rate to be 0.001 per bp in accordance with published records (Kwiatowski et al., 1991).
Consequently, the nested RT-PCR by Taq polymerase is expected to generate errors during cDNA synthesis (6.0×10−5 per bp) and PCR amplification (38.5×8×10−6 per bp), which sums to ∼36.8×10−5 per bp (i.e., one error in 2,717 bps).
A greater number of indels per bp sequenced was observed in the proximal promoters (1/1220) than in the 5' flanking regions (1/1885 bp).
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The effective sequence length (without Ns) in each window was used as the denominator to calculate per-bp values.
Previously published estimates of the population per-bp mutation rate (θW) (Hyten et al. 2006) were used to estimate the effective population of soybeans.
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