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DNA was extracted from one plant per accession using the MATAB method ([Risterucci et al. 2000]).
One plant per accession was grown in the greenhouse for DNA extraction.
On average, 230,813 SNPs per accession were detected, which means that 0.6 SNP was found per one kb of Nipponbare genome (382 Mb).
In each experiment, 10 individual plants were grown per accession in a completely randomised greenhouse design.
At both sites, seeds (three to four per accession) were sown directly in the soil.
Each individual's selfed progeny (five per accession) was planted at Sadore ICRISAT field station in June 2007.
The plants were distributed over two tables in a randomized design with one plant per accession on each table.
In 2004 and 2005, the number of weedy plants out of 25 plants per accession was assessed by two independent observers.
A hundred seeds per accession were placed in a petri dish and the number of seeds that had germinated after three days was counted.
Morphological data of five randomly chosen plants per accession were recorded individually in 2004 and 2005, and of 15 plants in 2007.
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Flexible regular expression-based search and replace classes scanning the sequence entries for necessary modifications are stored on a per-accession basis to curate the available organellar genomes.
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