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Copy number variations left after exclusion criteria were confirmed by MLPA using two probes per aberration.
The number of measurements per aberration mode P was chosen equal to 5 since this has proven to be an optimal value [ 7].
The number of photons for the aberration maps presented in this article was on average ≈ 10 photons per aberration mode and per subregion, resulting in a sub-region size L of about 10 20 μm.
We first define the non-centrality parameter as 2 where μ̂1 is the estimate of the ratio pertaining to the copy-number variation, μ̂0 the mean of the autosomal ratios, σ̂ the standard deviation, estimated using the autosomal targets, and n the number of adjacent targets per aberration.
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On average, we found more than one chromosomal aberration per abnormal mitosis (Fig. 3C).
A log-linear relationship was observed between the frequency of chromatid aberrations (excluding gaps) and the level of survival after CHIP treatment, with approximately one aberration per cell corresponding to 37% survival.
The mean male aberration frequencies per cell after HDR irradiation were 0.38 dic/cell and 0.61 TAb/cell; following LDR irradiation, the mean aberration frequencies were 0.28 dic/cell and 0.45 TAb/cell.
The median aberration number per case (number of chromosomes with at least one abnormal segment) showed a wide range, from 0 (squamous skin neoplasias, thyroid carcinomas) to 12 (small cell lung carcinomas; SCLC).
Treatment of mice with GA at 100 mg/kg bwt resulted in significant decrease in the percent aberrant cells and number of aberrations per cell compared to the control irradiated group.
In general, papillary tumors displayed very simple karyotypes with a modal number of 46 chromosomes per sample, and an average of 2.4 aberrations per tumor.
As expected, HG tumors were generally more affected than LG: average of 40.3 aberrations per tumor in HG versus 9.2 per tumor in LG (Additional file 3: Table S2).
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