Sentence examples for per μl of input from inspiring English sources

The calculated number of specific transcripts were normalized to the housekeeping genes cyclophilin B (CPB) and hypoxanthine guanine phosphoribosyltransferase (HPRT), and expressed as the amount per μl of input cDNA, as described previously [ 28].

Preamplification was performed using 3 μL of input RNA with PreAmp kit (Applied Biosystems).

Values were thus given as input adjusted copy number per μl of cDNA.

Values were thus given as input adjusted to the copy number per μl of cDNA.

The LF productions are presented as LF peak area per μL of supernatant.

The number of fragments per μl input purified circulating DNA (C Vi ) was calculated from the number of positive droplets P, total number of droplets analyzed T, droplet volume V d (0.91 × 10−3 μl), ddPCR volume V r (including PCR mix, primers, probe, input DNA), and volume of purified circulating DNA input into the reaction V i, using the formula.

A measure of 300  μl of the input was saved for RNA and protein analysis.

We used eight wells of 1 × TE/10 × Mg2+, resulting in a transfer volume of 125 nL per well per 10 μL of final volume.

Lysates were prepared at concentrations of 1000 and 10 000 cells per 50 μL device input volume.

The total transfer volume was 1.5 μL per 10 μL of final volume for a target of 50 nM.

A volume of 950 nL was transferred for a target concentration of 250 nM per 10 μL of final volume.