To facilitate permeabilization and mRNA probe penetration, samples were incubated in a freshly prepared 20 μg/mL proteinase K in PTw for 5 min, followed by washes in a filter-sterilized 10 mg/mL glycine solution.
Compared with other technical methods, drilling & coring, only utilizing the compound locomotion of rotation and penetration, can sample the subsurface soil relatively efficient and convenient.
High-energy short-wavelength synchrotron radiation allows penetration of samples of several centimeters of thickness.
During each electrode penetration, we sampled neuron activities at every 100- to 300-μm interval, and tested the color shape selectivities.
Furthermore, antibodies in their native form are unstable under certain conditions like elevated temperatures, and they have a large size that limits their penetration into samples and restricts their use in some applications.
When temperature is increased above 55 °C, density and viscosity of the extracts were decreases, which facilitate the solvent penetration deeper into sample matrix [27].
Increasing the incident beam peak energy would give greater penetration through the sample, but would have knock-on effects on the resultant diffraction pattern.
The fast exponent of the signal decay (~1 min) is absent (see Fig. 5), because the process is almost entirely controlled by slow rate of oxygen penetration into the sample.
It is because the penetration of the sample molecules is a slow process, and they find sufficient time to leach deep into the ground to a depth of 12 in.
It offers real progress in science with its intrinsic three-dimensional resolution, the absence of background fluorescence, and the attractive possibility of exciting UV-excitable fluorescent molecules, thus, increasing sample penetration.
