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It is because the penetration of the sample molecules is a slow process, and they find sufficient time to leach deep into the ground to a depth of 12 in.
The reason for this is most likely tissue autolysis, because thickness of the cancer specimens used for fixation was generally more than 2 cm, which implies more than 24 hours for penetration of the sample by the formalin [10].
Primary criteria were i) the full penetration of the sample by the staining agent and ii) the absence of artifacts.
After penetration of the sample, X-rays were converted into visible light by a thin Ce-doped YAG scintillator screen (Crismatec Saint-Gobain, Nemours, France).
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In addition, the penetration of the sampler increases with an increase in the inside diameter of the cutter, but decreases with an increase in the cutting angle.
The results show that the DSF can improve the mechanical properties and chloride ion penetration resistance of the sample.
As ultrasonication time increased, the dye penetration of the MTA samples also increased in an approximately linear manner (r = 0.446, P < 0.001).
To improve the antibody penetration of the tissue, samples were washed three times with 0.1% Tween-20 (Sigma) in PBS (PBST) for 5 minutes, digested with 20 μg/ml proteinase K in PBS for 30 minutes, and post-fixed in 4% PFA for 10 minutes.
Dry and saturated unit weights, water absorption by weight, effective porosity, capillary water absorption, slake durability index, P-wave velocity, point load index, uniaxial compressive strength and nail penetration index of the samples were determined.
After incubation time, at the end of the test, the samples were irrigated to field capacity water content, and penetration resistance of the samples was determined by penetrometer device (33-T0165 model, CONTROLS Inc).
It is illustrated through application on polymeric design objects corpus; (ii) the second is based on a barrier coating approach which strengthens the sample and avoids the penetration of the resin into the sample.
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