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The vacutainer tubes were stored on ice for no more than 60 minutes pending sample processing.
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In a few weeks, if pending samples test negative for the virus, India will be officially regarded as free from polio for the first time in its history.
The method requires 24 to 48 hours for confirmed detection of Campylobacter spp. (versus at least 72 hours for the NMKL method), enabling identification of positive flock lots to obtain retail product samples prior to distribution (two cartons of ready-to-ship broiler carcasses were held pending caecal sample results for another component of the full project).
We tentatively consider interbreeding between morphotypes within the Indo-Pacific clade unlikely as well, pending additional sampling and molecular data.
Such difference in the results, pending on sampling and/or techniques used, is an acknowledged concern in the field (Pritchard et al, 2012).
The puzzling absence of SUT-1 homologs in T. spiralis and T. muris means that such an interpretation must be provisional, pending broader sampling of genome sequences from other enopleans.
Based on the failure or success of PCR amplification, the size and position of each deletion fragment was deduced and new primers spanning estimated deletion fragment were designed for straightforward amplification of pending test samples (see schematic diagram in Figure 2).
Visualization of a 450 bp PCR product band in the MY09/MY11 lane and/or a 150 bp (190 200 bp when the GP6/MY11 heminested PCR or the HiFi nested PCR primers were used) band in the nested PCR lane provided presumptive evidence of an HPV DNA in the sample, pending genotyping by direct DNA sequencing for final validation.
Abbott et al. [ 5] also suggest that ND6 may be evolving in concert in Thalassarche albatrosses, but similarly acknowledge that this conclusion is tentative pending further individual sampling.
The institutional REC may grant provisional approval for transfer of samples pending the submission of both the MTA and provisional approval of the HREC to the NHREC who will acknowledge its receipt for record purposes only.
However, we note that Prolecithophora and Fecampiida are also both among the most minimally sampled higher 'turbellarian' taxa in this analysis, and that our sampled fecampiid has the longest inferred branch length of any non-neodermatan flatworm; this result should therefore be regarded as provisional, pending further taxon sampling of both clades.
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