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During a clinical outbreak of influenza at the boar station between 1st April 2011 and 31st July 2011, nasal swabs and blood samples were collected from a total of 375 of these pigs (three pigs per pen) to test for presence of virus and antibodies against the virus, respectively.
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Twenty-eight days after the 14 day old lambs were dosed with positive brain homogenate, four unchallenged lambs were mixed in the pens with the lambs to test for lateral transmission.
Use doodling to test old pens.
When something comes out, make swirls to test that the pen is working.
I asked for and received a pen to take home and test and was hooked from the first demo.
Other behaviors (pacing, circling, nibbling weeds (defined as 'displacement activity'), interactions with the objects or buckets ('irritability'), pawing, bleating, defecation, urination ('anxiety') or leaving the test pen to interact with the operator ('checking') were also recorded for all discriminations.
Fibroblasts were cultured in DMEM with 10 % FBS and 1 % strep/pen to 80%% confluency prior to testing.
SS mRNA levels in the PeN were tested by in situ hybridization.
Six groups of dogs from two facilities (total n=46) were observed in the home pen and tested for mechanical pressure threshold.
At follow-up, the ophthalmologist using a bright LED pen torch tested for the Relative Afferent Pupil Defect (RAPD).
If the pig still had not performed a correct run after 10 trials, it was placed back in the home pen and tested again one hour later.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com