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Novel floating pellets were prepared using the hot-melt extrusion (HME) technology.
The Enalapril Maleate (EM) pulsatile release pellets were prepared using extruding granulation, spheronization and fluid-bed coating technology.
Pellets were prepared using the standard technique under a pressure of 10 ton/cm2 with a barrel of 16 mm in diameter.
The melt pellets were prepared using lactose and polyethylene glycol (PEG) 3000 as hydrophilic filler/binder, calcium acetate and calcium carbonate as electrolytes, tolbutamide as poorly water-soluble drug in free form and embedded in PEG 3000.
Treatment pellets were prepared using Elvax 40P (provided by Dupont, Wilmington, DE, USA) as previously described (Silberstein and Daniel 1982).
Cell pellets were prepared using a lysosome enrichment kit (Pierce, Cat no. 89839) and transferred to a ball-bearing cell homogenizer (Isobiotec) and homogenized on ice as described in detail [ 16].
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Pellets containing solid dispersion (PSD) were prepared using optimal parameter settings demonstrated 88.52 ± 0.69% of the drug was released in a sustained release manner tili 12 h.
Pellets made of alginate and calcium salts were prepared using a solvent-free melt pelletization technique that prevented reaction between processing materials during agglomeration and allowed such a reaction to occur only in dissolution phase.
The samples were prepared using KBr pellets, and spectra were recorded at a resolution of 4 cm−1.
Total lysates were prepared using cell pellets from 1 ml aliquots of culture, which were resuspended in 100 μl ice-cold PBS and sonicated (3 × 10 s).
Finally the pellet was homogenized in the minimum volume of HBSS and the slides were prepared using 3 μL of the pellet.
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