Sentence examples for pellets were generated from inspiring English sources

For 37 ml of virus filter concentrate, virus pellets were generated by ultracentrifugation in a SW28 rotor (Beckman-Coulter, Fullerton, CA) at 100,000 g through a 1 ml 20% glycerol cushion and then virus was resuspended in 400 μl of PBS and layered onto a 9 step 24% to 56% sucrose gradient.

Cellular pellets were generated from ThinPrep material and subjected to Raman analysis.

For each patient (n = 4), two pellets were generated using each of the above mentioned cell concentrations.

When M. isabellina was cultured with NDLH, the pellets were generated within 24 hours, but the sizes of pellets were different between the treatment methods.

Briefly, cell pellets were generated, resuspended in 200 μL of 1X sodium dodecyl sulfate (SDS) buffer (0.1 M Tris HCl pH 6.8, 2 % β-mercaptoethanol (w/v), 2 % SDS (w/v), 10%% glycerol (w/v)) and boiled for 15 min.

A pellet was generated by centrifugation and the supernatant was discarded.

A cell pellet was generated by centrifugation and the recombinant protein was solubilized using a buffer containing non-ionic detergent.

Following this, cells were detached from the culture plates as for passaging, using standard Trypsin/Versene solution, and pellets from each well were generated after centrifugation at 1,000 rpm for 5 min at room temperature.

Purified stage 8 nuclei were generated by pelleting endogenous nuclei from stage 8 extracts multiple times through ELB buffer (250 mM sucrose, 50 mM KCl, 2.5 mM MgCl2, and 10 mM HEPES pH 7.8).

Spheroplasts were generated by digesting pellets with 950 µl 2 mg/ml Zymolyase 100T (MP Biomedicals) for 6 minutes.

Since proteomic profiles were generated from cell pellets growing on soluble sugars, detection of this enzyme class may be limited due to presence in culture supernatants only or possible lack of expression in the absence of lignocellulosic material [ 12].