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The cell pellets were collected and plasmid extraction was performed using QIAprep Spin Miniprep Kit (Qiagen, Germany).
Fresh faecal pellets were collected to measure cortisol metabolites and assess diet.
For the recovery experiment, following CCCP exposure cell pellets were collected by centrifugation and washed with LB and incubated again at 37 °C for 1 h.
Flagella pellets were collected by centrifugation (16,000g, 15 min, 4 °C), washed twice in PEME and resuspended in Laemmli buffer23.
The bacterial cell pellets were collected and subjected to cytoplasmic extraction with 20 mg/mL lysozyme followed by 4 min sonication on ice.
Further pellets were collected and supernatants were discarded.
The mycelium pellets were collected by centrifugation and then frozen in liquid nitrogen immediately.
Fungal biomass, grown in the form of pellets, were collected and washed five times with distilled water.
E. coil cell pellets were collected, resuspend in buffers, and repassed through the homogenizer at 700 bars.
Then, the samples were centrifuged for 10 min at 8000 rpm, and the cell pellets were collected.
The pellets were collected and washed with double distilled water to remove any unbound poloxamer-188 and free drugs.
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