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Total RNA was extracted from cell pellets using an RNeasy Mini Kit (Qiagen,Valencia,CA) for the BSL2 viruses.
Total RNA was extracted from cell pellets using an RNeasy mini Kit (Qiagen) and reverse transcribed using M-MLV prior to SYBR green qPCR as detailed in Methods S1.
The second approach involved pelleting a known number of cells from each individual culture, mixing cell pellets, then performing DNA extraction on the mixed pellets using an enzymatic DNA extraction (referred to throughout as "Enz").
Total RNA was isolated from cell pellets using an RNeasy Mini Kit (Qiagen, Hilden, Germany).
Total carotenoids were extracted from cellular pellets using an acetone extraction method [ 55].
Total RNA was isolated from cell pellets using an RNeasy minikit (Qiagen, Carlsbad CA).
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After collection, the filters were compressed into pellets using a hydraulic press device.
All infrared spectra were obtained from samples in KBr pellets using a Bruker Tensor27 Ger FT-IR spectrophotometer (Ettlingen, Germany).
Genomic DNA extraction was performed from the plasma-free blood pellets using a standard proteinase K and salt precipitation method.
Fourier transform infrared spectroscopy (FTIR) spectra were recorded with KBr pellets using a Rayleigh WQF-510 FTIR.
Total DNA was extracted from frozen cell pellets using a FastDNA® SPIN Kit for soil (MP Biomedicals LLC, Illkirch, France).
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