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For instance, if we restored normoglycemia using insulin pellets and thereby decreased β-cell proliferation, removing the pellets rapidly resulted in both hyperglycemia and increased β-cell proliferation (Figure 4B, "pellet-rem").
Implanted WA pellets rapidly oxidized and had a slightly eroded appearance.
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Copepods graze on the phytoplankton and release the carbon in fecal pellets that rapidly sink.
After centrifugation, the cell pellets were rapidly re-suspended in 1.2 ml warm Ringer's solution with 100 µM GABA and/or 100 µM picrotoxin, and incubated for 10 minutes.
The pellets were rapidly frozen in liquid nitrogen and stored at -80°C until further use.
The remaining algal pellets were rapidly frozen and lyophilized (Labconco lyophilizer, Kansas City, MO) for 48 h and stored at −20 °C for subsequent lipid analysis.
Samples were centrifuged at 700 × g for 10 min at 4°C, plasma was discarded and hemocyte pellets were rapidly frozen in liquid nitrogen before storage at -80°C.
Cell pellets were rapidly lysed by sonication and binding to Ni/NTA resin occurred, however most of the hexahistidine-fusion protein was found in the flow-through, indicating poor binding (data not shown).
Harvested cells were centrifuged at 1,500 g for 5 min, washed in phosphate-buffered saline (PBS), and pelleted at 1,500 g for 5 min. The cell pellet was rapidly frozen in liquid nitrogen and stored at −80°C.
The NPCP fraction in the cell pellet increased rapidly on the first day.
The pellet was rapidly frozen in liquid nitrogen and stored at −80 °C until RNA isolation.
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