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The starting material used as target submerged in de-ionized water consisted in pellets obtained from calcined fish bones.
Yeast cell pellets obtained from the pre-cultures were centrifuged (4000 rpm, 5 min, 4 °C), re-suspended and washed with 0.9% NaCl.
Yeast cell pellets obtained from the pre-cultures were centrifuged (4000 rpm, 5 min, 4 °C), resuspended and washed with sterile saline solution (0.9% NaCl).
Study Design: Genomic deoxyribonucleic acid and total ribonucleic acid were extracted from cell pellets obtained from the residual fluid-based Papanicolaou specimen collection buffer after clinical processing.
The electrical conductivity results obtained for these gadolinia-doped ceria and samaria-doped ceria ceramics are similar to those obtained for CGO pellets obtained from commercial nanopowders (Rhodia).
The total protein concentration from the collected 1 mL cell pellets obtained from the overall cultures was determined using the bicinchoninic acid (BCA) assay from Pierce (Rockford, USA) and protein expression was further analyzed using 15%% SDS-PAGE.
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The pellet obtained from the rinse specimen was re-suspended in 1 ml of sterile phosphate buffered saline.
The cell pellet obtained from human subcutaneous adipose tissue was resuspended in 10 ml of erythrocyte lysis buffer (0.154 M NH4Cl, 5.7 mM K2HPO4, and 0.1 mM EDTA, pH 7.3) and centrifuged.
The cell pellet obtained from a H. pylori CCUG 17874 50 ml culture was washed in 10 ml PBS, resuspended in 1.5 ml of 10 mM Tris-HCl pH 7.6, 20% sucrose and, after a 5 min incubation on ice, mixed with 50 µl of 0.5 M EDTA, pH 8.0.
The cell pellet obtained from this cGMP isolation step was homogenised in RIPA buffer with added protease inhibitor cocktail (Sigma Aldrich, Gillingham, UK), and the amount of total protein in the sample quantified using a Lowry-based colorimetric protein assay performed in triplicate (DC protein assay kit, Bio-Rad, Hemel Hempstead UK) compared to a bovine serum albumin standard curve.
The cell pellet obtained from 50 ml culture were re-suspended in 6 ml TriReagent (Sigma).
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