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The pellets, comprised of ZrC-coated UC particles (∼850 μm in dia).
Washes were pooled to the filtrate and centrifuged at 22,000 x g for 30 min. The pellets, comprised of free, non-viable organisms and host cell mitochondria, were resuspended in PBS, transferred to microfuge tubes and re-pelleted at 21,000 x g for 30 min. Supernatants were removed completely and the pellets were processed immediately or stored at −20°C.
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We illustrate the technique by means of the well-known problem of simultaneous diffusion and adsorption in a spherical sorbent pellet comprised of spherical, non-overlapping microparticles of uniform size and investigate the uptake dynamics.
The supernatant was filtered through 20 and 11 μm filters twice, respectively, and centrifuged at 4,000 rpm at 4 °C for 15 min. The pellet, comprised of smaller cells, was resuspended in 300 μl of PBS and 50 μl of 10× DNAse buffer and 5 μl of DNAse I were added and incubated at room temperature for 30 min to digest any DNA not contained in whole cells.
Pellets comprising paracetamol, which is highly soluble in ethanol, showed alcohol dose dumping regardless of the matrix composition.
In contrast, pellets comprising calcium stearate as single matrix component showed robustness in alcoholic media due to wettabilities that were not affected by the addition of ethanol.
Pellets comprising chitosan, cellulose microcrystalline, povidone, filler excipient and diclofenac sodium as model drug were prepared by extrusion spheronisation.
If the remaining food pellet comprised more than 0.09 g/10 g BW (2 SEM of the average food intake of normal food; see Results section) on day 2, the mice were not used for the in vitro experiments.
We call the device a flux controlled pump, and it is comprised of compressed pellets of a mixture of drug and hydroxypropyl cellulose within the hollow core of the ring.
In pellets, the collagen network was comprised of single fibrils with diameters ranging from 20 to 30 nm.
Equal amounts of protein were incubated with FOXO3 (Cell Signaling) or p53 (Santa Cruz) antibodies at 4°C overnight, and the complexes comprised of DNA-protein were pelleted with protein G-agarose.
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