The supernatant was discarded and the pellets air-dried.
The supernatant was carefully removed and the pellets air-dried at room temperature in the dark.
The supernatant was removed and the pellet air dried for 5 minutes.
Following a final centrifugation at room temperature for 10 minutes at 13,000 rpm, ethanol was carefully removed with a pipette and the RNA pellet air dried.
Proteins were pelleted, air dried for 1 h, and lysed in 7 M urea−2 M thiourea−4% CHAPS-30 mM Tris-HCl (pH 8.1) by rocking for 1 h at ambient temperature for subsequent labeling.
The supernatant was discarded, the pellet air dried and re-suspended in 30 μl of RNAse free water.
After a short spin the supernatant was removed completely and the pellet air dried at room temperature.
Protein was precipitated in ice cold acetone at -20°C overnight, pelleted by centrifugation and the pellet air dried to remove residual acetone.
The supernatant was then discarded again and the pellet air dried for 10 min and afterwards re-suspended in 100 μl RNase-free water (dH2O).
The supernatant was removed by pipette, and the RNA pellet air dried for 5 min prior to addition of 25 μl of RNase free water.
