Sentence examples for pellet of cells from inspiring English sources

Tissue pieces were gently agitated and pellet of cells was resuspended.

After centrifugation at 800 g, the pellet of cells was frozen in liquid nitrogen.

The pellet of cells was resuspended in 0.1% sodium citrate, 0.1% Triton X100 and 50 µg/ml propidium iodide (PI), and then stored for 24h at 4°C.

After treatment with isotonic or hypotonic solution for 15 min, a pellet of cells was formed by rapid centrifugation of the cell suspension at 80 g.

The pellet of cells was washed with 10 ml of PBS at pH7.2 and 10 µl of 0.5 M EDTA (final conc. 0.5 mM) and filtered though a sterile cheese cloth to remove any debris.

The 1 ml pellet of cells was washed in 10 to 20 ml of TD buffer (134 mM NaCl, 5 mM KCl, 0.7 mM Na2HPO4, 2.5 mM Tris-HCl, pH 7.5) and centrifuged for 5 min at 2.5 krpm in a Sorvall centrifuge.

M protein marker; 1 purified SBP-Cap∆41 protein; 2 pellet of cell lysate after sonication and centrifugation; 3 supernatant of cell lysate after sonication and centrifugation; 4 lysates of pET-28a expressing cells.

Proteins were analyzed in washed cells and in the supernatant or pellet of cell lysates.

The root tissue extract was ground, passed through a 60-μm sieve, and subsequently centrifuged at 500 g for 5 min to obtain a pellet of cell debris.

Equal amount of supernatant from the pellet of cell culture medium were loaded onto 4 12% SDS-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (Bio-Rad laboratories, Hercules, CA, USA).

For experiments requiring ectopic expression of CLN3, SC medium was pre-depleted by growth of sch9Δ cells for 7 days followed by pelleting of cells and filter sterilization of medium.