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The suspension was carefully homogenised and ultracentrifugated to pellet down the viral nucleocapsids.
The cells from both the +ve and the –ve fractions were pellet down and the cell pellets were suspended in KO medium.
Cells were pellet down by centrifugation (1200 rpm for 5 min) and 70% ethanol was added dropwise into the cell pellet (1 5×107 cells) followed by incubation (−20° for 2 hours).
The sample was centrifuged at 1000 g for 15 min at 4°C and 1-ml aliquots of the supernatant was transferred to microfuge tubes and spun at maximum speed to pellet down any remaining debris.
Centrifugation was done to pellet down DNA.
(C ) Samples were ultracentrifuged to pellet down the purified membranes, which sat on top of a transparent solid Percoll layer.
Similar(46)
After which, UCNPs-anti-HSA antibody was pelleted down and washed thrice with DI water by centrifugation at 13,000 rpm for 15 min prior to further use.
The cells were pelleted down, centrifuged at 2,000 rpm for 10 min at 4 °C, and washed with Annexin V-FITC binding buffer (10 mM HEPES, 140 mM Nacl, and 2.5 mM CaCl2, 2H2O; pH 7.4).
Next, the solution of carboxyl-modified microbeads was collected in a 1.5 mL low-adhesion tube (Bio-plastics Singapore) and pelleted down under centrifugation at 6000 rpm for 2 min.
After this, the UCNPs were pelleted down and washed three times with DI water under centrifugation at 13,000 rpm for 15 min and resuspended in DI water by pipette mixing and sonicated for 30 s.
Cells were pelleted down, washed twice with cold PBS, and finally dissolved in PBS treated with RNase A for 30 min at 37 °C and stained with propium iodide (20 μl from 50 μg/ml) and kept in the dark for 15 min.
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