Four punches from each specimen and cell pellet controls were placed on separate blocks as previously detailed [ 17, 18].
Initially, the ability of clone 28 to recognize activated c-Src using the IHC protocols above was confirmed using HCT116 cell pellet controls.
The pump orifice size and chemistry of the polymer pellets control the rate of hydration and diffusion of the drug-containing hydroxypropyl cellulose gel from the device.
In Exp. 1, naturally infected lambs (n = 76) were weaned (102.7 ± 1.4 d of age) in May springg) and randomly assigned in a 2 × 2 factorial design to receive 2% of BW/d of alfalfa pellets (control) or SL with or without amprolium added to drinking water (n = 38/level or 19/treatment).
The chevon was then used to prepare two types of pellets (control or enriched chevon) that were then fed to twenty-male-four-month-old Sprague-Dawley rats (n = 10 in each group) for 12 weeks to evaluate their effects on plasma cholesterol levels, tissue fatty acids, and gene expression.
At 7 (exp 1) or 10 (exp 2) weeks of age mice were implanted with 120-day 17β-oestradiol pellets or control pellets (Innovative Research of America, Sarasota, FL, USA).
Formalin-fixed paraffin sections of breast cancer tissue and cell pellets (controls) were mounted on 3-aminopropyl-triethoxysilane-coated (Sigma, Poole, Dorset, UK) glass slides and were baked for 30 min at 56 60°C before being dewaxed in Clearene (Surgipath Europe, Peterborough, UK).
Compared to the calcium-phosphate-treated serum pellets, the control untreated pellets gave comparable dark field images except for the much smaller amounts of precipitate material seen (Fig. 2A and E).
Sovová's broken-and-intact cell model was applied to a selection of pellet and control substrate extraction curves to determine a microstructural factor (MF = De/D12) as best-fitting parameter.
HL-1 myocytes were incubated under cyanide/aglycemia during 60 min followed by 5 min centrifugation and re-suspension of the cell pellet in control buffer for simulated reperfusion.
Mice were then fed group-specific pellet diets (control; Western) (Ssniff GmbH, cat. no. E15000-04 and E15741-34, respectively).
