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This paper reports a solution for commonly used pellet cells, which mitigates these problems.
Ni/CGO CGO LSCF/CGO three electrode pellet cells at 500, 550 and 600 °C were exposed to dry carbon monoxide for fixed periods of time, at open circuit and under load at 50 and 100 mA cm−2, in an aggressive test designed to accelerate electrode degradation.
We performed the following sequential action: (1) the cells detached with trypsin and EDTA; (2) centrifugation at 750×g for 5 min; (3) washing the pellet cells with PBS containing calcium; (4) cells were re-suspended in 100 μl binding buffer; (5) cells were mixed with 2 μl annexin V-FITC; and at the end, (6) 2 μl PI was added to mixture and the flow cytometric analysis was done.
Plates were centrifuged for 3 min to pellet cells, and FACS buffer was removed.
Pellet cells in pre-cooled tumblers at 4°C, for 10 15 min at 4000×g.
The samples were centrifuged to pellet cells and the cells were resuspended in SDS-PAGE loading buffer.
After overnight growth, 1 ml of each induced culture was centrifuged at 10K rpm for 1 min. to pellet cells.
Cells were spun for 5 minutes at 8000 rpm to pellet cells using a Microfuge 18 benchtop centrifuge (Beckman Coulter).
The pellet cells were washed once in L15, and resuspended in advanced DMEM-F12 medium containing 1% N-2 supplement and 1% penicillin/streptomycin.
At 5 hours all well contents were transferred to a 1.5 ml Eppendorf and spun for 10 min at 1000RPMs to pellet cells.
Briefly, 4 ml aliquots of O157 H7 cultures were centrifuged at 3200×g, 4 °C, for 10 min to pellet cells.
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