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Western blot analysis of the pellet cell fraction of lysed E. coli strains.
Hemolymph samples were centrifuged at 4 °C and 12,000g for 10 min to pellet cell debris.
We centrifuged the media at 3000×g for 15 min to pellet cell debris.
The lysates were spun at 12,000×g at 4°C for 5 min to pellet cell debris.
Following centrifugation at 8,000×g for 5 min to remove cells, the supernatant was centrifuged at 40,000×g for 1 h to pellet cell membrane material.
Following centrifugation to pellet cell debris, lysates were mixed with 100 µl immobilized avidin beads (Pierce) and rocked at 4°C for 1 hour.
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This paper reports a solution for commonly used pellet cells, which mitigates these problems.
Plates were centrifuged for 3 min to pellet cells, and FACS buffer was removed.
Pellet cells in pre-cooled tumblers at 4°C, for 10 15 min at 4000×g.
The samples were centrifuged to pellet cells and the cells were resuspended in SDS-PAGE loading buffer.
Cells were spun for 5 minutes at 8000 rpm to pellet cells using a Microfuge 18 benchtop centrifuge (Beckman Coulter).
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