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Also, these conditions resulted in a higher percentage of the pellet being in the main size fraction (0.800 1.250 mm) and a larger time required to dissolve 63.2% of the drug (τd).
4ml samples were taken into 1% phenol 19% ethanol (final v/v), incubated at 4°C for 45 minutes, spun down at 3320g for 10 minutes at 4°C before the pellet being stored at −80°C [2].
For analysis of lead concentration in relation to the quantity of spent ammunition visible on X-rays of the carcass, we used the number of pellet equivalents, with all fragments greater than 10% of an intact pellet being summed (the example given above would be scored as 1.6 pellets).
At 30 minutes post-infection or control, 10ml samples were taken into 1% phenol 19% ethanol (final v/v), incubated at 4°C for 45 minutes, spun down at 3320g for 10 minutes at 4°C before the pellet being stored at −80°C for later RNA purification [2].
Bacteria were then incubated protected from light either at room temperature for 90 min or at 37°C or 42°C for 30 min. Excess dye was removed by eight consecutive washes with 1 mL of PBS, with the final pellet being resuspended in 1 mL of PBS.
This resulted in the majority of the fecal pellet being dissolved and filtering of the sample was not performed.
Similar(51)
The pellet is later regurgitated.
The remaining dry pellet is dropped.
If one pellet is missing, you will be killed.
The neutrophil-rich pellet was resuspended in 2 ml PBS.
Each pellet was washed once with 1 mL methanol.
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