We independently crystallized the complex under high PEG conditions similar to that reported by the Conti group [30], [44].
Since no significant difference had been observed by QCM-D between 5% and 15% PEG conditions, only coatings obtained with in-solution concentrations of PEG up to 5% were compared to LP and to bare PET surfaces.
Crystals of LanM were obtained by hanging drop vapor diffusion method, by mixing 1 μl of protein (concentration of 2 6 mg/ml) with an equal volume of precipitant of either 0.2 M CaCl, 0.1 M HEPES pH 7.5, 10 mM betaine hydrochloride, and 28% PEG 400 (condition 1) or 0.2 M KCl, 0.05 M HEPES pH7.5, 10 mM barium chloride, and 33% 5/4 PO/OH (condition 2).
In addition, the changes of H2O2, DHA and MDA content reflected positive effect of SA pretreatment in PEG stress condition rather than other indicators.
Under the four PEG stress conditions, from DAY 0 to DAY 3 POD activities increased progressively before dropping from DAY 4 to DAY 5 in the rehydration period.
The two potato types were allocated to three drought treatments of no stress - normal water condition, PEG 10% (moderate stress) and PEG 20% (severe stress); each treatment has 15 replications arranged into a completely randomized design.
Needle- and blade-like crystals were obtained in multiple PEG-containing conditions, and these hits were optimized using finer screens.
Under the complete coverage of the surface condition, PEG molecules are in direct competition for the adsorption sites on the AuNP surface [18].
Two type flower-like SnO2 hierarchical architectures with ultrathin nanosheets as building blocks have been controllably synthesized via tuning the presence or not of PEG under hydrothermal condition.
Under these conditions, PEG 400 has the highest bulk measured viscosity, glycerol and TEG have similar viscosities, and sorbitol has the lowest, as shown in Fig. 8C.
