Notably the induction profiles differed across the cell types; in the EC cell line HIF-1α levels peaked already at 2 h whereas in ACs peak induction occurred at 6 h.
The times-to-peak, however, were different: WT mice achieved peak induction of all three transcription factors by 24 hours PI, whereas the TLR2−/− mice peaked by 48 hours PI.
The results showed peaked micronuclei induction by mitomycin C and bleomycin (clastogens) at the 12 h/12 h schedule, while colchicine and vinblastine (aneugens) showed the strongest effect at 24 h/0 h.
However, only a few time points were analyzed in the microarray analyses, and so it is difficult to determine the exact time at which gene expression peaked after induction by BPH.
Most genes in this study peaked in their induction or repression by 2 h.
SIRT1 RNA and EGR1 protein induction peaked between 3 and 4 hours (Fig. 1a and 1b).
Under drought stress in root, transcript induction peaked within 24 h, but in leaves it was seen at 48 h of drought stress.
In addition, RANKL, a marker of osteoclast differentiation and activation, was elevated from 24 hours after arthritis induction, peaked on day 4, and had fallen to control levels again by day 14.
The CCR2 induction peaked the first time within 24 hpt (26.67-fold 26.67-fold) and thexpressionime within 48 hpt (41.65-fold expression) before being decreandd athe2 hpt (2.01-fold expresecond.
In Min6 cells, active caspase-3 was detected after 2 h of hypoxia treatment, and the induction peaked at 8 h, indicating that commitment of Min6 cells to apoptosis occurs after a short period of exposure to hypoxia.
