It is unclear, whether or not corrections for peak summing of Cs γ-photons were performed in the γ-spectroscopy, so some uncertainty may have been introduced here.
In case of a complex mixture with unknown number of components, number of peaks, sum of resolutions and retention time of ultimate peak were considered as output variables.
For each peak the sum of the intensities for the three strongest isotopic signals was calculated and normalized against the sum for all the Aβ peaks in the spectrum, followed by averaging of results for separately determined duplicate samples.
Although DNA concentration was controlled during the preparation of the plates, for data analysis each sample was normalized internally by dividing the height of each individual peak by total sum of peaks heights (dosage quotient).
Normalized peak heights were calculated by dividing the peak height of each peak by the sum of the heights of all peaks.
> -wrap-foot> The response values from the individual runs (24 total) were used for ANN-GA analysis a−1 and +1 indicate low and high value for the respective factors Cholesteryl ester separation was evaluated for the total number of peaks, the retention time of the last eluting peak, and the sum of all peak resolutions, as described in Novotna et al. [ 15].
R was defined as the ratio between the distance of peak values and the sum of peak widths at half height, with the second peak as reference.
The program determines the percent of cells within each peak, and the sum of all peaks in the control culture is taken as 100% of proliferation and 0% of suppression.
The program determines the per cent of cells within each peak, and the sum of all peaks in the control culture is taken as 100% of proliferation and 0% of suppression.
Briefly, the MI is determined by comparing the ratio of each peak to the sum of the total peaks and then calculating the difference between sum of the longer Aβ peptides (e.g., Aβ42) and that of the shorter Aβ peptides (e.g., Aβ37, Aβ38, and Aβ39).
