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There was a significant enrichment for OCT4 motifs for a peak score of 0.44 and above.
Following those criteria an arbitrary eVAAS peak score of 50 was used as threshold to identify the responders (mean of the eVAAS peak scored during AIR+2 SD).
By taking the genomic positions of the identified peaks as a reference (that is to a peak score of at least 0.5), we assembled the sub-sequences underlying the peaks.
For a peak score of 0.33 and above, 15 of the 16 OCtargetgenesenes were previously reported as having critical roles in both mouse and human ES cells [23].
However, mice co-injected with dihydrokainate (MOG+ceftriaxone+DHK) exhibited an indistinguishable clinical course with a peak score of 2.8±1.4 (p = 0.80) and a residual score of 1.2±0.2 (p = 0.51; Fig. 5A; Tab. 1).
MOG-immunized mice treated with ceftriaxone (MOG+ceftriaxone) again displayed a significantly attenuated clinical EAE course with a reduced peak score of 3.0±0.6 (p<0.01 **) 17 days after immunization and a residual score of 1.5±0.5 (p = 0.01 *; Fig. 5A; Tab. 1).
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Firstly, the peak scores of each activity are added up to others.
The ICED is derived from the peak scores of the Index of Disease Severity and the Index of Physical Impairment using an algorithm specific to the ICED.
Our analysis revealed a strong positive correlation with regard to gene upregulation and peak scores of the activating histone modifications and PolII.
To investigate if most of our targets contain an octamer motif, we screened all the peak regions of 497 target genes for OCT4 motifs, using a peak-score of 0.5 and ranked them based on a significance score.
In callpeaks, we define a peak score in terms of a Poisson P-value, which is calculated independently for each chromosome, and in terms of one peak property, either the peak height or read count.
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