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The G1 peak of reference strains was located at positions 31.6 (CBS8710) and 31.1 (CBS10510), and the G2 peak was located at positions 65.8 (CBS8710) and 56.4 (CBS1056.4.
Within the algorithm, cross correlation peak of reference image and image to be registered is estimated from classical Fourier domain method with the upsampling factor of 2 for inverse FFT step.
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However, the method suffers from the lack of chemical information with peak assignments based on the retention times of authentic standards or known peaks of reference materials.
Because peak heights vary depending on sample, base type and their position within the sequence, the PeakPicker software carries out a normalization step in which the SNP allele height is compared to the height of reference peaks in flanking sequence.
The software divides the normalised peak areas of the patient by the average peak areas of reference samples (four reference samples from healthy individuals were introduced in each run).
Peak heights for each probe were intrasample normalized using the peak heights of reference probes in the same sample.
Full width at half maximum (FWHM) for a diffraction peak of this reference sample at the 2θ = 43.38° was 0.129°; therefore, it made it possible to distinguish anatase and brookite phases.
According to the chromatographic peak of monosaccharides reference substance and samples, the composition of the monosaccharides in PHP was identified.
When one of the alleles of the analysed fragment undergoes deletion, its peak decreases and, at the same time, the peak of the reference amplicon increases (Fig. 1a, red curve).
The situation is reversed in the case of amplification, with the peak of the reference amplicon decreasing and the peak of the analysed amplicon increasing (Fig. 1a, blue curve).
Instrument settings (voltage and gain) were adjusted to allow for fluorescence intensity of expected ploidy levels, together with the peak of the reference plant, to be located between channels 250 750 on the x-axis (using a 1024 scale) of plots of counts of nuclei vs FL2A.
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