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Religious leaders reassured people suspicious about vaccinations, and politicians knocked on doors to make sure children took their medicine.At the peak of coverage, 99.7% of the target population swallowed anti-polio drops, says Anuradha Gupta of the national health ministry.
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In contrast, TAP-treated libraries showed discontinuous read distributions with peaks of coverage commonly occurring upstream of annotated genes.
We then asked whether HDRs were indeed causing false positive peaks of coverage in high-throughput molecular biology assays.
As expected, there is a relationship between GC content and coverage, but this effect is too small to account for the dramatic peaks of coverage we see in the data (Supplementary Fig. S5).
As shown in Figure 4 (green line), the peak depth of coverage for the transcriptome is 1.45×.
All coverage plots we have generated show a peak of high coverage between 8,000 9,000 bp, which roughly corresponds to the location of atp8.
This finding is strikingly different compared with nuclear genome sequences obtained using Illumina technology, where the peak of sequencing coverage typically occur at a GC content close to 50% e.g. [ 34, 35].
In particular, a peak of increased coverage depth in the 300 bps following the repeat was observed in affected family members (II:3, III:1 and III:2) but not in non-affected members (II:2 and III:3) (Fig. 3A).
These CapFilter results include a non-negligible portion of low-coverage peaks (10 20 reads), highlighting its ability to capture true TSS peaks regardless of coverage.
Using this cap-signature filter is especially effective because 1) it does not require a priori genome annotation, and 2) it applies to all peaks, regardless of coverage or position along a gene's sequence.
Some peaks of high coverage were evident in the untreated library.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com