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After adding polyvinyl alcohol fibers, the stress-crack opening relationship of the composites changes to a double peak mode behavior as the crack bridging first decreases from cracking strength, then increases to the second peak.
This property allows us to clearly divide signals for each peak (mode).
However, both results had a distinct peak (mode) at pI 4.2.
In peak mode ITP, trace analytes do not appreciably contribute to local conductivity.
The motif search was applied to ±25 nucleotides around the peak mode.
To filter internal priming, we removed PA clusters with 15 'A' in the 20 nucleotides region downstream of peak mode.
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The limit of detection of the method was 14 pg ml−1 of mercury, using peak area mode while LDs was 1.3 pg ml−1 using peak height mode, with a 2% RSD and a linear dynamic range of almost three orders of magnitude.
We then calculated the median and average distances between corresponding peak modes for all pair-wise comparisons.
The average distance between peak modes ranged from 12 to 17 nucleotides, with 10 to 12 nucleotides after G'-filtering.
Only those peaks which could be paired uniquely were used, and the median and average distance between the identified peak modes was calculated.
To address the reproducibility of our nanoCAGE-XL data, we examined the correlation in read coverage for individual peaks, the consistency between TSS peak modes (the position most commonly sequenced in a read cluster) of biological replicates, and the consistency between nanoCAGE-XL peak modes and those in a PEAT data set generated using the same sample type [ 3].
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