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(B) MALDI-TOF MS of the peak fraction of PXL2LRR-peptide shown in (A).
The scCO2 treatment gave rise to increase in peak fraction of free ClO4− anions (620 625 cm−1) and peak shift of ν(C O C) mode to lower frequency region (1060 1070 cm−1) depending on ether Li+ interactions.
The larger molecular size peak fraction of β-catenin reflects β-catenin/α-catenin dimers, as this peak perfectly cofractionates with α-catenin (Fig. 2A), shRNA knock-down of α-catenin eliminates this size fraction of β-catenin (Fig. 2B & C), and α-catenin is the major binding partner of β-catenin in these cells (Fig. 2D).
The peak fraction of the protein was collected for EM studies.
$$\end{document} f E = fraction of aptamer sequences in complex peak fraction of aptamer sequences in original injected signal.
The best fit occurred with a defect size of ∼0.18 µm (d in Fig. 16 ▶ b) with a diffuse peak to kinematical peak fraction of ∼0.5.
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Right: Coomassie blue staining of the peak fractions of PXL2LRR and SERK2LRR shown in (F) following SDS PAGE.
Right: Coomassie blue staining of the peak fractions of PXL2LRR shown in the left following SDS-PAGE.
As an example, Fig. 2 A shows the eluted peak fractions of DDX3wt from the last column (HAP).
In accordance with this, we found some γ-secretase activity in the Golgi-enriched fractions in the iodixanol gradient although the peak fractions of γ-secretase activity did not correlate with GM130 expression and high γ-secretase activity in Golgi/trans-Golgi network has earlier been observed in different cell lines [3], [4].
Peak fractions of the chromatograms were analysed by SDS-PAGE and silver staining.
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