Clustering analysis also revealed large groups of coordinately expressed genes with peak expression in AF (Cluster 4 with 792 transcripts, Figure 3D and Additional file 4) or in AM (Cluster 5 with 985 transcripts, Figure 3E and Additional file 4).
The expression of another B-class gene, AP3, was also found to be specific to reproductive tissues in Arabidopsis; however its rice ortholog, OsMADS16, also expressed in vegetative tissues besides showing peak expression in reproductive tissues.
In poly I C-injected fI C-injectedtranscripts showed peak expression in the spleen, intestine and heart at 12 h post-injection (hpi).
However, the only increases in transcript expression were shown in the comparison of Y vs G and R vs G in cellulase (CEL3 and 5) and beta 1,4-glucanase (such as transcript 6), with a peak expression in the yellow stage.
By contrast, three AP2yfp fusion proteins had peak expression in parasites containing internal daughters.
NvClock showed strong diurnal expression with peak expression in the mid- to late subjective day (Figure 1B, zeitgeber time (ZT) = 6 12).
CD152 exerts its main function during its peak expression in a T cell response, which is typically 48 h following activation [11], [16].
Intriguingly, the TRP2 element is similar to the DNA binding site recently determined for the putative transcription factor AP2XI-3 (Gissot and Kim, personal communication), which had a peak expression in the G1 phase (Table S1).
Cell cycle and bradyzoite developmental AP2s are largely separate gene groups with the exception of AP2VIIa-1, whash has peak expression in tachyzoite cytokinesis and is also substantially increased under bradyzoite induction conditions in vitro (Behnke and White, unpublished).
Thus, overall, the MET3pr displays fast turn on and off times, has rather low cell-cell variability, and has peak expression in a physiological range at least as judged by the comparison to CLN2pr.
Together, the pattern of rhythmic expression for these two bHLH-PAS transcripts was more similar to Drosophila, where DmClock exhibits peak expression in the light portion of a light:dark cycle, albeit at an earlier portion of this period (ZT = 0 4) [28], [28].
