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UGT activity was also significantly rhythmic with nearly two-fold difference between peak enzyme activity at ZT20, and minimum activity at ZT16 (Figure 2B).
For all species, specific enzyme activity was minimal at time 0, and peak enzyme activity could occur at any other time point, depending on the enzyme and fungal species.
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Drug treatment caused an acute stimulation of ornithine decarboxylase in fetal and neonatal lung and blunted the peak of enzyme activity seen at 20-30 d postnatally without affecting acute responsiveness to beta-adrenergic stimulation (isoproterenol).
Kinetic analysis of A-SMase activity revealed a peak of enzyme activity at 2 min after agonist addition, indicating that the enzyme acts as a downstream effector of the receptor.
After the column was washed with five column volumes of buffer B, the enzyme was eluted with a linear gradient of 0 0.5 M imidazole in buffer B. The peak of enzyme activity eluted at an imidazole concentration range of 0.06 0.27 M.
The peaks in enzyme activity after 15 days (β-glucosidase) were similar to those found in a previous study with pig manure processed by E. fetida [26], [27], although the presence of earthworms produced greater stimulation of the enzymatic activities.
Tissue extract prepared from a CMT patient (No. 23) was applied on the Superose 12 column and around 70% of total TK1 activity eluted as major peak with MWs of 40 100 kDa, but with a minor peak and some enzyme activity in the fractions corresponding to higher MW.
Native cellular TK1 activity eluted as major peak with molecular weights (MWs) of 40 66 kDa, but with a small peak and some enzyme activity in the fractions at the higher MW range.
For GBSS1, the peak values of enzyme activity were formed during 15 20 days after flowering.
For soluble starch synthase, the peak values of enzyme activity were showed at 10 days after flowering.
For starch branching enzyme, the peak values of enzyme activity were formed during 10 15 days after flowering in six varieties except for Palgong and Samnam.
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