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By plotting the conditioning voltage vs the peak current amplitude at −60 mV, we calculated that removal of inactivation had a V50 of 85.5 ± 5 mV (n = 10).
Electrophysiological experiments indicate that mitral cell neurons cultured from both heterozygous and homozygous-null mice (IR+/− and IR−/−) have an decreased peak current amplitude compared with that recorded for wild-type animals matched for days in vitro (DIV).
The rotor peak current amplitude varies from 3.83 to 6.4 A and from 7.8 to 9 A respectively in the two cases.
We used only cells that produced a peak current amplitude <5 nA.
Indeed, we show here that increasing the stimulus magnitude increases the peak current amplitude for both SA and Transient currents.
The figures illustrate effects of gluconate replacement on different channel combinations and plot the inhibition of peak current amplitude.
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For E161K, the cell surface signal was reduced to ∼70%, but peak current amplitudes were reduced to <30% (Fig. 6).
The current-voltage relationship revealed that peak current amplitudes at +20 mV were significantly reduced following coexpression of Kv11.1-wt and Kv11.1-mut constructs (Figure 4B).
SEW2871 facilitated Icaps, and peak current amplitudes transiently increased in a dose-dependent manner with the maximum effect occurring at 1 µM (2.46±0.50 fold, p<0.05, n = 6; Wilcoxon matched pairs test; Fig. 4D).
As measured by the ratio between the sustained and the peak current amplitudes, the macroscopic inactivation is 45±3% (n = 13) and 19±2% (n = 7) for L233W and Q244W, respectively (Figure 4B and D).
Inward peak current amplitudes were measured.
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