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Cell population doubling time (PDT) was calculated using the following equation: PDT (hr) = (log2 × t)/(logNt - logN0), where t = time in culture (hr), Nt = final cell count, N0 = original cell count.
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The Caspase 3/7 activity was the highest at 12 hrs compared to those at 8 and 16 hrs after PDT, and the LDH activity in the cytotoxicity assays was higher at 1 hr than that at 4 hrs after PDT.
Van der Veen et al. [ 19] also observed a reduction at 4 hr and 10 hr post-PDT using the same methods in UVB-treated skin models (100 mW/cm, 100 J/cm).
However, for PTSCs and ATSCs at later passages (> 12), the PDTs were increased to 187 ± 20.2 hr and 236 ± 40.1 hr, respectively, indicating that these cells were in a senescent state.
Henderson et al. observed that ALA induced blood perfusion reduction using fluorescein methods in Colon-26 tumor (75 mW/cm, 135 J/cm) at 1 hr, 3 hr, 5 hr, and 24 hr post-PDT [ 18].
The photochemical treatment (i.e. PDT) is known to induce a rapid cell inactivation causing cell death within 24 hrs after light exposure [24].
PDT on June 13.
alone (without PDT).
Lysosome disruption after PDT.
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