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Therefore, the circadian abnormality in pdf >hAPP flies (Rezával et al., 2008) is probably unrelated to toxic Aβ peptides.
Because many aspects of Vasoactive intestinal polypeptide (VIP) function in mammals resemble those of PDF in flies (Vosko et al., 2007), it will be interesting to know if VIP cell firing can mimic light and phase-shift mammalian rhythms.
Figure 1A includes a representative actogram of progressively older heterozygous pdf-gal4 flies bearing a single copy of the driver employed in the genetic screen.
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Like pdf 01 mutant flies, a mutant in PDFR (pdfr 5304 flies) exhibits a reduced morning peak and a phase advanced evening peak in LD as well as a short period and a high percentage of arrhythmic flies in DD (Hyun et al., 2005; Lear et al., 2005).
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The expression of nuclear β-galactosidase in the four classes PDF neuron in flies with various Gal4/uas-LacZnls combinations was imaged using a LUMPL 60×/1.10 water objective with correction collar.
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