Sentence examples for pd buffer from inspiring English sources

The beads were washed three times with 1 ml of PD buffer and then resuspended in 2X SDS sample buffer.

For GST-14-3-3 GST-14-3-3 GST-14-3-3 GST-14-3-3ce with ice-cold pulldownshen lysed in 1 ml of 14-3-3 pulldown buffer (PD buffer; 15 mM Tris, pH 7.5, 150 mM Nacells.5% NP-40, 1 mM DTT) supplemented were a protease inhibitor tablet (Roche).

Prior to the growth of Co/Pd multilayers, a 10-nm-thick Pd buffer layer was grown on the Si substrate and AAO templates.

The [Co(2 Å)/Pd(10 Å)]15 multilayers were then deposited on the Pd buffer layer by an e-beam system with a growth pressure of around 5 × 10-8 Torr.

NadA, LOX-1 and the complex mixed in a 1 1 molar ratio were diluted in HBS-P buffer in a final concentration of 5 μM.

For kinetics analysis of the interaction between CRT (>90% pure) and immobilized ITGA4 peptide, 40 μL of various concentrations of CRT in HBS-P buffer were injected at a flow rate of 20 μL/min.

The isolated RCs were slurry suspended in a 0.01-M Na-P buffer, pH 7.2, with 0.05% LDAO [8].

Separated RCs were suspended in a 0.01-M Na-P buffer with a pH of 7.2, which contained 0.05 % LDAO [18].

Cells were washed once with 10.3% sucrose and resupended in P buffer containing 2 mg/ml lysozyme [34].

The running buffer was HSB-P buffer (10 mM HEPES [pH 7.4], 150 mM NaCl, 0.005% polysorbate 20 adjusted to 1 mM MgCl2) (BIAcore).

Injections on the anti-Fc coated CM5 chip were done at a flowrate of 5 µl/min and the different proteins were diluted in HBS-P buffer.