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Many broad-specificity enterovirus real-time reverse transcription PCRs target conserved regions of the 5′-UTR.
Because these 2 PCRs target different DNA segments, the positive PCR results were not due to PCR contamination with amplicons.
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Three multiplex polymerase chain reactions (PCRs) targeted on Bifidobacterium and related species were designed to identify human species.
Polymerase chain reactions (PCRs) targeting Chlamydia pneumoniae, Chlamydia.
For PCRs targeting the contiguous loci, only the first locus is shown.
For those PCRs targeting gaps of ~900 bp or greater, the long PCR reaction and thermoprofile was used (see above).
PCRs targeting W22703-specific genes tcaA, tcaC and two genes of Flg-2 were performed as a further control.
When both PCRs targeting the second and the third gene were negative, the result was classified as negative.
PCRs targeting the stx1 and stx2 (4, 5 ) eae (6 ), and ehxA (7 ) genes were performed as described.
Using the Clustal Multiple Alignment Algorithm, we identified a highly conserved 245-bp PCR target on the above seven tetracycline resistance genes (Table 1).
Each qRT-PCR target was measured with 5 replicate wells for each sample (n = 5).
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