Our finding that dogs have a higher seroprevalence and are positive by PCRs supports the hypothesis that they, rather than cats, might be the main mammalian reservoir of R. felis [ 14].
These findings, together with the easier implementation of commercial EIAs than qRT-PCRs, support the use of EIA for identifying cases and controls to estimate vaccine effectiveness, even though a few rotavirus infections might be missed by the EIA, particularly in specimens for which Ct values are high (low viral loads).
JV participated in primer/probe design and provided real-time PCR support.
Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.
We especially wanted to find out how well FISH, IHC, and real-time RT-PCR support the sequencing results.
Instead of full genomes resulting from exchange of sequence between the parental genomes, rearranged (deleted) sequences that were recovered by PCR support this recombination event.
Each single DNA molecule of the library is bound to a bead that acts as PCR support, and amplified within an aqueous droplet.
These TSP assay configurations for allele-specific PCR support rapid, low-cost endpoint SNP genotyping, since they are not reliant on fluorescently-labeled primers or probes and they do not require individual assay optimisation to accurately assign a genotype.
The results from all the analyzed genes by qRT-PCR support the significant differences of mRNA expressions detected by microarray analysis although the relative fold change of transgenic mice to wild-type mice did not match exactly.
Data from ESTs and hybridization experiments in addition to our transcriptome and expression analysis by RT-PCR support the existence of two distinct KLC gene groups, the zygotic AaKLC2-like genes, and the highly conserved AaKLC1-like genes that are found in all mosquitoes and insects surveyed.
For these latter 6 targets, we confirmed their differential expression by quantitative RT-PCR (Supporting Dataset S2).
