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None of the 24 six-plex PCRs amplified five or more SNP loci, and only half of the four-plex assays amplified three or more SNP loci (Table 1).
Sequencing of the various amplicons generated from the 11 primer pairs confirmed that all PCRs amplified their target sequences.
Both PCRs amplified E. histolytica (8 ).
Three independent, semi-nested PCRs amplified the Igγ variable genes and two independent nested PCRs amplified Igκ and Igλ variable genes.
The two multiplex PCRs amplified a total of 13 loci in the L genome and 13 loci in the R genome.
Sixteen (67%) of the 24 six-plex PCRs amplified five or more SNP loci, and all of the 24 four-plexes amplified three or more SNP loci.
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RT-PCR amplified preferentially HevPBANR-C from female pheromone glands.
All captured genomic DNA was bisulfited converted, PCR amplified and sequenced on the Illumina HiSeq 2500.
The cmx transposon was PCR amplified from P. aeruginosa PA7 by primer xj143 (as both forward and reverse primer).
Inserts for cloning were PCR amplified from MM1.S genomic DNA using the primer pairs provided in ref. 20.
The DNA extraction was automated performed and the STR loci was PCR amplified using PP17 kit.
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