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For all multiplex PCRs, amplification was performed in a GeneAmp PCR 9600 DNA thermal cycler (Perkin Elmer, Waltham, MA, USA).
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Forward primers were then added for the subsequent PCR amplification.
(D) PCR amplification using primers flanking the target site.
However, the possibility that PCR amplification may be observed in mutated clones cannot be eliminated.
Genotyping of poor metabolisers of debrisoquine by allele-specific PCR amplification.
After PCR amplification, the PCR products were purified with Qiagen Purification Kit (Hilden, Germany).
PCR amplification of TCR β-chain CDR3s was performed as described above.
Details of DNA extraction and PCR amplification are listed in Supplementary Materials and Methods.
Gel electrophoresis was performed to confirm that no PCR amplification bands existed.
PCR amplification was performed with primers Prok_340F and Prok_806R as described46.
PCR amplification was carried out using Pfu Turbo DNA polymerase (Stratagene).
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