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Only PCR with Pwo yielded PCR products.
PCR with CV -80% yielded only slightly increased bias estimates.
Subsequently, detected pathogens were quantified by PCR with specific primers.
We then secondarily amplified all samples using PCR with primer designed for STR domains.
Figure 2 is an illustration of multiplex PCR with three forward primer and three reverse primers.
PCR with these primers resulted in an IL8 promoter fragment of 250 bp (NCBI NM 000584).
The mda-7/IL-24 gene was obtained by PCR with the primers.
Mice were genotyped by using PCR with the mouse tail DNA as a template.
OsRap2.6 RNAi plants were screened using PCR with Rap2.6 primers listed in Table 2.
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(A) The expression of TLR4 was quantified by RT-PCR with purified DCs.
Hence, we could not correlate RT-PCR with disease severity or treatment response to ATT.
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