Sentence examples for pcr used from inspiring English sources

RNA was isolated and poly(A) PCR and real-time PCR used to measure expression levels of 30 genes.

The same outer PCR was used as template both for 10 separate inner PCRs for Pyrosequencing as well as an inner multiplex PCR, used for PrASE.

It was not possible to subtype all AIVs because the conventional PCR used for subtyping was not as sensitive as the surveillance qRT-PCR.

Conclusions: The Roche LC assay was more sensitive than the nested PCR used in this study.

Conclusions: The herpes consensus PCR for a given virus was more sensitive than the standard, targeted PCR used in our laboratory.

The method is based on the polymerase chain reaction (PCR) used in conjunction with restriction fragment length polymorphism (RFLP) analysis and comprises three parts.

PCR used an initial denaturation of 94°C for 5 min, followed by 40 cycles of 94°C for 45 sec, 38-43°C 38-43°Csec and 72°C for 90 sec.

The SSRs, which usually have a higher mutation rate, are easily genotyped using PCR, used as markers for phylogenetic analysis and in marker-assisted breeding when located on nuclear chromosomes.

All PCR used Phusion Hot start (Finnzyme, Finland).

A first round of PCR used primers generated by Primer3 [29] to amplify from genomic DNA samples.

Thermal asymmetric interlaced PCR (TAIL-PCR) is a nested PCR used for amplification of unknown template DNA fragments [61] [64].