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As long as high molecular weight DNA is not required like in RFLP approaches, DNA extracted from formalin fixed and paraffin embedded tissues can be made suitable for polymerase chain reaction (PCR) analysis significantly by virtue of its sensitivity of detection (by PCR usage) and an ability to analyze minute samples, provided standardized extraction protocols are strictly followed.
Table 2 TrustCAM PCR usage.
Table 2 summarizes PCR usage of the TrustCAM prototype.
Though there is widespread DNA typing acceptance in forensic science field importantly due to its sensitivity of detection (by PCR usage) and an ability to analyze minute samples.
Finally, a set of results is presented as a typical example of comparative real-time PCR usage; the potential and limitations for this approach are addressed.
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To our knowledge, this is the first report of microarray and quantitative reverse transcription PCR (qRT-PCR) usage associated with semigamy and will hopefully lay the groundwork towards understanding its genetic mechanism, regulation and control.
More recently, using real-time PCR, biased Vβ usage and CDR3 sequences were shown in the synovial fluid T-cell repertoire of RA patients, particularly in patients with HLA-DRB1*0405 [ 57].
Using RT-PCR, we demonstrated usage of both promoters E and F in monocytes and macrophages, consistent with expression of ERα66 and ERα46.
In a few cases, no amplification was obtained after decreasing the initial annealing temperature of the touch-down PCR and adapting codon usage.
Full length HOS2 gene was amplified by using 4 different primers (Table 1) using splicing by overlap extension (SOE PCR), so that codon usage could be maintained in any heterologous expression system.
An mRNA-based HLA-DR monitoring by polymerase chain reaction (PCR) would improve the clinical usage and facilitate conduction of large multicenter studies.
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