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The FastPfu PCR system (TransGen) was used in PCR.
qPCRs were performed with SYBR Green quantitative PCR master mix (Toyobo Co). on a quantitative PCR system (7900HT Fast Real-Time PCR System, Applied Biosystems).
In the normal PCR and multi-PCR system, the specific PCR products were achieved when the different concentrations of QDs were added into the PCR system.
Amplification was performed in Takara PCR System (Takara, Japan).
The PCR system was applied to isolates from fungemia patients.
Quantitative real-time PCR (qRT-PCR) was performed using TaqMan real-time PCR system (Thermo Fisher Scientific) and SYBGreen real-time PCR system (TaKaRa).
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and an Mx3000P Multiple Quantitative RT-PCR system (Stratagene; La Jolla, CA).
Reverse transcription was performed by using ThermoScriptTM RT-PCR system.
Real-time PCR Amplification was performed using the Applied Biosystems 7900 HT fast RT-PCR system.
cDNA was constructed using ThermoScript RT-PCR system (Invitrogen, Carlsbad, CA) following manufacturer's instructions.
We used the Superscript one-step RT-PCR system from Invitrogen.
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