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The main finding of this study is that touchdown general primer PCR coupled with control of the quantity of sample DNA in a PCR supports the detection of low-copy number HPV in an excess background of human DNA sequences.
Although a positive PCR supports the diagnosis of neuroborreliosis, a negative PCR does not exclude it [ 18].
Real time quantitative PCR supports previous microarray results showing COX5A is expressed in cerebral cortical tissue at a higher level in human than in chimpanzee or gorilla.
Thus, experimental validation using strand-specific PCR supports the validity of our empirical criteria for the selection of tissue-specific antisense expression.
We also demonstrate that multiplex-ready PCR supports an improved level of multiplexing in plant genomes of varying size and ploidy, without the need to carefully optimize assay conditions.
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The quality and abundance of short nascent strands in the DNA preparations was assessed by real time PCR (Supporting Figure S2).
JV participated in primer/probe design and provided real-time PCR support.
Our approach to establishing susceptibility to infection was to use quantitative PCR supported by immunofluorescence testing.
Expression analysis for APX by Real-Time PCR supported this result.
Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.
Instead of full genomes resulting from exchange of sequence between the parental genomes, rearranged (deleted) sequences that were recovered by PCR support this recombination event.
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