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JV participated in primer/probe design and provided real-time PCR support.
Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.
Instead of full genomes resulting from exchange of sequence between the parental genomes, rearranged (deleted) sequences that were recovered by PCR support this recombination event.
Each single DNA molecule of the library is bound to a bead that acts as PCR support, and amplified within an aqueous droplet.
These TSP assay configurations for allele-specific PCR support rapid, low-cost endpoint SNP genotyping, since they are not reliant on fluorescently-labeled primers or probes and they do not require individual assay optimisation to accurately assign a genotype.
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The quality and abundance of short nascent strands in the DNA preparations was assessed by real time PCR (Supporting Figure S2).
Our approach to establishing susceptibility to infection was to use quantitative PCR supported by immunofluorescence testing.
Expression analysis for APX by Real-Time PCR supported this result.
Although a positive PCR supports the diagnosis of neuroborreliosis, a negative PCR does not exclude it [ 18].
Real time quantitative PCR supports previous microarray results showing COX5A is expressed in cerebral cortical tissue at a higher level in human than in chimpanzee or gorilla.
We collected single dlg5 D48 embryos to map the breakpoints of the deletion created by the P-element excision by PCR (Supporting Information, Figure S1).
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